gene exp amigo2 hs05001325 s1 (Thermo Fisher)
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gene exp amigo2 hs05001325 s1
Gene Exp Amigo2 Hs05001325 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amigo2/Gene+Exp%2E+AMIGO2%2C+Hs05001325_s1/pmc12623045-40-80--1
Average 94 stars, based on 1 article reviews
Gene Exp Amigo2 Hs05001325 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amigo2/Gene+Exp%2E+AMIGO2%2C+Hs05001325_s1/pmc12623045-40-80--1
Average 94 stars, based on 1 article reviews
gene exp amigo2 hs05001325 s1 - by Bioz Stars,
2026-10
94/100 stars
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1) Product Images from "Increased HSD11β1 Expression in Human Leiomyomatous Uteri: Implication for Enhanced Glucocorticoid Signaling"
Article Title: Increased HSD11β1 Expression in Human Leiomyomatous Uteri: Implication for Enhanced Glucocorticoid Signaling
Journal: The Journal of Clinical Endocrinology and Metabolism
doi: 10.1210/clinem/dgaf255
Figure Legend Snippet: Silencing of FKBP5 blunts dexamethasone (DEX)-regulated expression of glucocorticoid response element-containing genes in primary leiomyoma cell cultures. qPCR analysis of mRNA levels for DEX-upregulated genes TSC22D 3 , ADH1B, IL1R1 (A), and DEX-downregulated genes GREM1, IGFBP1, AMIGO2 (B) in cultured leiomyoma cells transfected with either control or FKBP5 siRNA for 48 hours and then treated with either vehicle (Cont) or DEX for 24 hours. Bars represent mean ± standard error of the mean, n = 4.
Techniques Used: Expressing, Cell Culture, Transfection, Control
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Article Title: Increased HSD11β1 Expression in Human Leiomyomatous Uteri: Implication for Enhanced Glucocorticoid Signaling Article Snippet: The full name and Taq-Man Probe Assay IDs for each target gene include: FK506-binding protein 5 ( FKBP5 )- Hs01561006_m1; Hydroxysteroid 11-β dehydrogenase 1 ( HSD11B1) - Hs01547870_m1; IL-1 receptor type 1 ( IL1R1 )- Hs00991002_m1; TSC22 domain family member 3 ( TSC22D3 )- Hs00608272_m1; alcohol dehydrogenase 1B, β polypeptide ( ADH1B )- Hs00605175_m1; Gremlin 1, DAN family BMP antagonist ( GREM1 )- Hs00171951_m1; IGF-binding protein 5 ( IGFBP5 )- Hs00181213_m1; adhesion molecule with Ig-like domain 2 ( AMIGO2 )- Hs05001325_s1; laminin subunit alpha 2 ( LAMA2 )- Hs00166308_m1; fibronectin 1 ( FN1 )- Hs00365052_m1; calponin 1 ( CNN1 )- Hs00959434_m1; actin β ( ACTB )- Hs99999903_m1; and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH )- Hs99999905_m1. 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Article Snippet: Taqman primers/probes as follows: AQP4 (Mm00802131_m1), SLC10A6 (Mm00512730_m1), NOX1 (Mm00549170_m1), C3 (Mm01232779_m1), SOD1 (Mm01344233_g1), AMIGO2 (Mm00662105_s1), GFAP (Mm01253030_1), GSTA2 (Mm00833353_mH), PRDX1 (Mm01621996_s1), 18 s rRNA (HS999999_s1). Recombinant:Article Title: Irisin reduces amyloid-β by inducing the release of neprilysin from astrocytes following downregulation of ERK-STAT3 signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 ChromPure Donkey IgG, whole molecule Jackson immunoresearch Cat# 017-000-003; RRID: AB_2337256 Chemicals, peptides, and recombinant proteins Paraformaldehyde Thermo Fisher Scientific Cat# AA433689L OneMinute Plus WB Stripping Buffer GM bioscience Cat# GM6015 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34096 RGDS peptide R&D systems Cat# 3498/10 SB273005 Selleck Cat# CS7540 10 His-tag irisin Lake Pharma Kim et al.23 Lysis buffer 6 R&D systems Cat# 895561 RIPA buffer Boston bioproducts Cat# BP-115 L-a-Aminoadipic acid Santa Cruz Cat# SC-202200 b-Amyloid (1-42), HiLyte Fluor 555 Ana Spec Cat# AS-60480-01 Halt Protease/ Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 U0126 Tocris Bioscience Cat# 1144 Corning Cell Recovery Solution Corning Cat# 354253 Corning Matrigel Basement Membrane Matrix Corning Cat# 354234 N-Lauroylsarcosine sodium salt Sigma-Aldrich Cat# L5777 Sacubitril calcium salt Sigma-Aldrich Cat# SML1380 Cal-520, AM AAT Bioquest Cat# 21130 IL-1a recombinant protein Sigma-Aldrich Cat# I3901 TNFa recombinant protein Cell signaling Technology Cat# 8902SF C1q recombinant protein Mybiosource Cat# mbs143105 IL-1b recombinant protein R&D systems Cat# 201-LB IFN-g recombinant protein R&D systems Cat# 285-IF Doxycycline Stemcell technologies Cat# 72742 Critical commercial assays V-PLEX Ab Peptide Panel 1 (4G8) Kit Meso Scale Discovery Cat# K15199E V-PLEX Ab Peptide Panel 1 (6E10) Kit Meso Scale Discovery Cat# K15200E V-PLEX Proinflammatory Panel 1 Human Kit Meso Scale Discovery Cat# K15049D Neprilysin Activity Assay Kit (Fluorometric) Biovision Cat# K487 Insulin Degrading Enzyme Activity Assay Kit Ana Spec Cat# AS-72231 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 miRNeasy Mini Kit Qiagen Cat# 217004 iScript gDNA Clear cDNA Synthesis Kit BioRad Cat# 1725035 SsoAdvanced Universal Probes Supermix BioRad Cat# 1725282 Promega CytoTox-ONE Homogeneous Membrane Integrity Assay Promega Cat# G7891 Irisin ELISA Phoenix Pharmaceuticals Cat# 067-29 BDNF ELISA R&D systems Cat# SBNT00 Deposited data Raw and processed data files for scRNAseq This paper NCBI-GEO: GSE240161 Raw and processed data files for mass spectrometry This paper iProX: PXD044499 (Continued on next page) Neuron 111, 3619–3633.e1–e8, November 15, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines ReNcell VM human neural precursor (ReN) cells EMD Millipore Cat# SCC008 Human iPSC-Derived Astrocytes Axol Bioscience Cat# AX0665 Oligonucleotides Nep (Hs00153510_m1) Life Technologies Cat# 4331182 Gfap (Hs00909233_m1) Life Technologies Cat# 4331182 Cp (Hs00236810_m1) Life Technologies Cat# 4331182 Cd44 (Hs01075864_m1) Life Technologies Cat# 4331182 C3 (Hs00163811_m1) Life Technologies Cat# 4331182 Serping1 (Hs00163781_m1) Life Technologies Cat# 4331182 Amigo2 ( shRNA:Article Title: Irisin reduces amyloid-β by inducing the release of neprilysin from astrocytes following downregulation of ERK-STAT3 signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 ChromPure Donkey IgG, whole molecule Jackson immunoresearch Cat# 017-000-003; RRID: AB_2337256 Chemicals, peptides, and recombinant proteins Paraformaldehyde Thermo Fisher Scientific Cat# AA433689L OneMinute Plus WB Stripping Buffer GM bioscience Cat# GM6015 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34096 RGDS peptide R&D systems Cat# 3498/10 SB273005 Selleck Cat# CS7540 10 His-tag irisin Lake Pharma Kim et al.23 Lysis buffer 6 R&D systems Cat# 895561 RIPA buffer Boston bioproducts Cat# BP-115 L-a-Aminoadipic acid Santa Cruz Cat# SC-202200 b-Amyloid (1-42), HiLyte Fluor 555 Ana Spec Cat# AS-60480-01 Halt Protease/ Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 U0126 Tocris Bioscience Cat# 1144 Corning Cell Recovery Solution Corning Cat# 354253 Corning Matrigel Basement Membrane Matrix Corning Cat# 354234 N-Lauroylsarcosine sodium salt Sigma-Aldrich Cat# L5777 Sacubitril calcium salt Sigma-Aldrich Cat# SML1380 Cal-520, AM AAT Bioquest Cat# 21130 IL-1a recombinant protein Sigma-Aldrich Cat# I3901 TNFa recombinant protein Cell signaling Technology Cat# 8902SF C1q recombinant protein Mybiosource Cat# mbs143105 IL-1b recombinant protein R&D systems Cat# 201-LB IFN-g recombinant protein R&D systems Cat# 285-IF Doxycycline Stemcell technologies Cat# 72742 Critical commercial assays V-PLEX Ab Peptide Panel 1 (4G8) Kit Meso Scale Discovery Cat# K15199E V-PLEX Ab Peptide Panel 1 (6E10) Kit Meso Scale Discovery Cat# K15200E V-PLEX Proinflammatory Panel 1 Human Kit Meso Scale Discovery Cat# K15049D Neprilysin Activity Assay Kit (Fluorometric) Biovision Cat# K487 Insulin Degrading Enzyme Activity Assay Kit Ana Spec Cat# AS-72231 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 miRNeasy Mini Kit Qiagen Cat# 217004 iScript gDNA Clear cDNA Synthesis Kit BioRad Cat# 1725035 SsoAdvanced Universal Probes Supermix BioRad Cat# 1725282 Promega CytoTox-ONE Homogeneous Membrane Integrity Assay Promega Cat# G7891 Irisin ELISA Phoenix Pharmaceuticals Cat# 067-29 BDNF ELISA R&D systems Cat# SBNT00 Deposited data Raw and processed data files for scRNAseq This paper NCBI-GEO: GSE240161 Raw and processed data files for mass spectrometry This paper iProX: PXD044499 (Continued on next page) Neuron 111, 3619–3633.e1–e8, November 15, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines ReNcell VM human neural precursor (ReN) cells EMD Millipore Cat# SCC008 Human iPSC-Derived Astrocytes Axol Bioscience Cat# AX0665 Oligonucleotides Nep (Hs00153510_m1) Life Technologies Cat# 4331182 Gfap (Hs00909233_m1) Life Technologies Cat# 4331182 Cp (Hs00236810_m1) Life Technologies Cat# 4331182 Cd44 (Hs01075864_m1) Life Technologies Cat# 4331182 C3 (Hs00163811_m1) Life Technologies Cat# 4331182 Serping1 (Hs00163781_m1) Life Technologies Cat# 4331182 Amigo2 ( Software:Article Title: Irisin reduces amyloid-β by inducing the release of neprilysin from astrocytes following downregulation of ERK-STAT3 signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 ChromPure Donkey IgG, whole molecule Jackson immunoresearch Cat# 017-000-003; RRID: AB_2337256 Chemicals, peptides, and recombinant proteins Paraformaldehyde Thermo Fisher Scientific Cat# AA433689L OneMinute Plus WB Stripping Buffer GM bioscience Cat# GM6015 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat# 34096 RGDS peptide R&D systems Cat# 3498/10 SB273005 Selleck Cat# CS7540 10 His-tag irisin Lake Pharma Kim et al.23 Lysis buffer 6 R&D systems Cat# 895561 RIPA buffer Boston bioproducts Cat# BP-115 L-a-Aminoadipic acid Santa Cruz Cat# SC-202200 b-Amyloid (1-42), HiLyte Fluor 555 Ana Spec Cat# AS-60480-01 Halt Protease/ Phosphatase Inhibitor Cocktail Thermo Scientific Cat# 78440 U0126 Tocris Bioscience Cat# 1144 Corning Cell Recovery Solution Corning Cat# 354253 Corning Matrigel Basement Membrane Matrix Corning Cat# 354234 N-Lauroylsarcosine sodium salt Sigma-Aldrich Cat# L5777 Sacubitril calcium salt Sigma-Aldrich Cat# SML1380 Cal-520, AM AAT Bioquest Cat# 21130 IL-1a recombinant protein Sigma-Aldrich Cat# I3901 TNFa recombinant protein Cell signaling Technology Cat# 8902SF C1q recombinant protein Mybiosource Cat# mbs143105 IL-1b recombinant protein R&D systems Cat# 201-LB IFN-g recombinant protein R&D systems Cat# 285-IF Doxycycline Stemcell technologies Cat# 72742 Critical commercial assays V-PLEX Ab Peptide Panel 1 (4G8) Kit Meso Scale Discovery Cat# K15199E V-PLEX Ab Peptide Panel 1 (6E10) Kit Meso Scale Discovery Cat# K15200E V-PLEX Proinflammatory Panel 1 Human Kit Meso Scale Discovery Cat# K15049D Neprilysin Activity Assay Kit (Fluorometric) Biovision Cat# K487 Insulin Degrading Enzyme Activity Assay Kit Ana Spec Cat# AS-72231 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 miRNeasy Mini Kit Qiagen Cat# 217004 iScript gDNA Clear cDNA Synthesis Kit BioRad Cat# 1725035 SsoAdvanced Universal Probes Supermix BioRad Cat# 1725282 Promega CytoTox-ONE Homogeneous Membrane Integrity Assay Promega Cat# G7891 Irisin ELISA Phoenix Pharmaceuticals Cat# 067-29 BDNF ELISA R&D systems Cat# SBNT00 Deposited data Raw and processed data files for scRNAseq This paper NCBI-GEO: GSE240161 Raw and processed data files for mass spectrometry This paper iProX: PXD044499 (Continued on next page) Neuron 111, 3619–3633.e1–e8, November 15, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines ReNcell VM human neural precursor (ReN) cells EMD Millipore Cat# SCC008 Human iPSC-Derived Astrocytes Axol Bioscience Cat# AX0665 Oligonucleotides Nep (Hs00153510_m1) Life Technologies Cat# 4331182 Gfap (Hs00909233_m1) Life Technologies Cat# 4331182 Cp (Hs00236810_m1) Life Technologies Cat# 4331182 Cd44 (Hs01075864_m1) Life Technologies Cat# 4331182 C3 (Hs00163811_m1) Life Technologies Cat# 4331182 Serping1 (Hs00163781_m1) Life Technologies Cat# 4331182 Amigo2 ( Gene Expression:Article Title: The spatial zonation of the murine placental vasculature is specified by epigenetic mechanisms. Article Snippet: The following TaqMan gene expression assays were used for murine samples: Mm00607939_S1 (Actb), Mm00483057_m1 (Cav1), Mm03053893_gH (Ccnb1), Mm01171453_m1 (Ccnb2), Mm00432359_m1 (Ccnd1), Mm01135198_m1 (Cd36), Mm01151063_m1 (Dnmt1), Mm00432881_m1 (Dnmt3a), Mm01240113_m1 (Dnmt3b), Mm00475056_m1 (Lyve1), Mm01278617_m1 (Mki67), Mm00448100_g1 (Pcna), Mm01242584_m1 (Pecam1/Cd31), Mm00435613_m1 (Pgf), Mm00477872_m1 (Uhrf1) and Mm01320970_m1 (Vecam1). .. The following TaqMan gene expression assays were used for human samples: Hs00962877_m1 (ACP1), Hs01060665_g1 (ACTB), Hs00969450_g1 (ADM), Article Title: Rapid Generation of Ventral Spinal Cord-like Astrocytes from Human iPSCs for Modeling Non-Cell Autonomous Mechanisms of Lower Motor Neuron Disease Article Snippet: .. Analysis of gene expression was conducted using the following oligonucleotide primers: Taqman probes FOXG1 , Hs01850784_s1; HOXA3 , Hs00601076_m1; HOXA5 , Hs00430330_m1; HOXB8 , Hs00256885_m1; NKX2.2 , Hs00159616_m1; NKX6.1 , Hs00232355_m1; NKX6.2 , Hs00752986_s1; PAX6 , Hs01088114_m1; PAX3 , Hs00240950_m1; PAX7 , Hs00242962_m1; GFAP, Hs00909233_m1 ; S100B , Hs00902901_m1; CD44 , Hs01075864_m1; GJA1 , Hs00748445_s1; SLC1A2 , Hs01102423_m1; SLC1A3/EAAT1/GLAST (EAAT1), Hs00904823_g1; AQUAPORIN-4 (AQP4), Hs00242342_m1; TFAP2A , Hs01029413_m1; REELIN , Hs01022646_m1; POTASSIUM INWARDLY RECTIFYING CHANNEL SUBFAMILY J MEMBER 10 ( KCNJ10 ), Hs00158426_m1; AMIGO2 , Expressing:Article Title: Central TYK2 inhibition identifies TYK2 as a key neuroimmune modulator Article Snippet: .. Target Genes Housekeeping Genes Model (Score) Gene (Catalog #) Species Gene (Catalog #) IFN Challenge Mouse Model (ISG Score) Rsad2 (Mm00491265_m1) Mouse Gapdh (Mm99999915_g1) Ifit3 (Mm01704846_s1) Hprt1 (Mm00446968_m1) Cxcl10 (Mm00445235_m1) Hmbs (Mm00660262_g1) Usp18 (Mm01188805_m1) Gusb (Mm00446953_m1) Oasl1 (Mm00455081_m1) Actb (Mm00607939_s1) EAE Mouse Models (ISG Score) Cxcl10 (Mm00445235_m1) Ipo8 (Mm01255158_m1) Isg15 (Mm01705338_s1) Pgk1 (Mm00435617_m1) Ifi30 (Mm00499131_m1) Rplp2 (Mm00782638_s1) Ifi35 (Mm01260550_g1) Tbp (Mm00446973_m1) Ifi44 (Mm00505670_m1) Tfrc (Mm00441941_m1) Ifit1 (Mm00515153_m1) Ubc (Mm01201237_m1) Ifit3 (Mm01704846_s1) Human Gapdh (Hs02786624_g1) Mx1 (Mm00487796_m1) Rpl36al (Hs00733231_m1) Oas1a (Mm00836412_m1) Slc25a3 (Hs00358082_m1) Human iPSCderived Astrocytes (Comprehensive and Reactive Scores) Amigo2 ( |
